Date of birth: October 6,1979
Department of Cellular Membranology
Bogomoletz Institute of Physiology
Bogomoletz 4, 01024, Kiev
Ukraine
Tel.(Fax): +380 44 256 2590
handly: +380 95 8706070
e-mail: vera@biph.kiev.ua

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Current project:

Investigation of non-hygrolizible diadenosine polyphosphate analogue modulation of synaptic transmission in hippocampal slices and NMDA-receptor channel properties in isolated CA3 pyramidal neurons.

 Diadenosine polyphosphates (ApnAs), where n is 2-6, are ubiquitous in Nature and found in both intracellular and extracellular locations. ApnAs have been identified as constituents of synaptic vesicles in the PNS or CNS, and previous evidence has suggested that ApnAs may exercise a variety of effects such as increasing the spontaneous action potentials in locus coeruleus neurons of pontine slices, raising the level of excitation in nodose ganglion neurons, as well as depressing extracellular postsynaptic field potentials and excitatory postsynaptic current (EPSC) to a similar extent (Klishin et al., 1994; Pintor et al., 2000) . Recent studies have indicated that high-affinity binding sites for Ap4A (Pintor and Miras-Portugal, 1995) , as differentiated from P1-or P2-receptors, are found in the olfactory bulb, cerebral cortex, and striatum, as well as several other brain areas (Rodriguez-Pascual et al., 1997) . These receptors have been identified as purinergic P4-receptors (Rodriguez-Pascual et al., 1997) . Taken together, these data suggest that diadenosine polyphosphates may use unique signal transduction pathways and may activate physiological responses different from those caused by ATP or adenosine. The ambiguities that surround ApnA function derive in part from the difficulty of handling ApnAs in that they are unstable to specific enzymic and non-specific hydrolysis in the presence of biological fluids and tissue samples (McLennan, 1992; Guranowski, 2000) . For this reason, we elected to revisit the role of Ap4A in neurotransmission using the non-hydrolysable analogue AppCH2ppA.

1. Fisyunov A, Tsintsadze V, Min R, Burnashev N, Lozovaya N. Cannabinoids modulate the P-type high-voltage-activated calcium currents in purkinje neurons J Neurophysiol. 2006 Sep;96(3):1267-77. Epub 2006 May 31
   
   
2. Melnik S, Wright M, Tanner JA, Tsintsadze T, Tsintsadze V, Miller AD, Lozovaya N. Diadenosine polyphosphate analog controls postsynaptic excitation in CA3-CA1 synapses via a nitric oxide-dependent mechanism. J Neurophysiol. 2006 Sep;96(3):1267-77. Epub 2006 May 31
   
   
3. В. П. Цинцадзе, А. Л. Федоренко, Т. Ш. Цинцадзе, М. Райт, Дж. А. Теннер, Э. Д. Миллер, Н. А. Лозовая. Действие негидролизуемого аналога диаденозин полифосфатов на НМДА-опосредуемые токи в изолированных пирамидных нейронах гиппокампа. Нейрофизиология. – 2006. – Т. 38, № 3, – С. 205-210.
   
   
4. С.І. Мельник, М. Райт, Дж.А. Теннер, Т.Ш. Цінцадзе, В.П. Цінцадзе, Е.Д. Міллер, Н.А. Лозова. Аналог діаденозинових поліфосфатів модулює передачу сигналу в зрізах гіпокампа. Фізіол. Журн. – 2006. – Т. 52, № 4, – С. 9-12. 
   
   
5. С.І. Мельник, М. Райт, Дж.А. Теннер, Т.Ш. Цінцадзе, В.П. Цінцадзе, Е.Д. Міллер, Н.А. Лозова. Модуляция активности синапсов в зонах СА1-СА3 гиппокампа аналогом диаденозин полифосфатов: роль оксида азота. Нейронауки. – 2005. – Т. 1, № 1, – С. 73-74.